10 x Laemmli Running buffer

250 mM Tris, pH 8.3    30.3 g
1 SDS                        10 g
1.92 M Glycine            144 g
H2O                   up to 1000 ml

Store at room temperature.

Chemicals:

  • Tris base, ‘Sigma’, T-1378, Fw 121.1 g/mol

  • SDS, ‘BDH’, No. 44244, Fw 288.38 g/mol

  • Glycine, ‘Merck’, No. 1.04201, Fw 75.07 g/mol

 

2003 Julius