Coomassie Blue staining
Staining solution:
0.1 Coomassie Brilliant Blue R-250 1 g
40 methanol 400 ml
10 acetic acid 100 ml
50 H2O up to 1000 ml
Filter the solution after 20 min of stirring. Store at room temperature.
To stain the proteins in polyacrylamide gels: after the electrophoresis, incubate the gels in the stain for > 45 min on the orbital shaker at room temperature.
The staining solution can be used many times before replacing.
Important! Sensitivity is about 100 ng! Coomassie stains the proteins through the gel!
Destaining solution:
40 methanol 400 ml
10 acetic acid 100 ml
50 H2O up to 1000 ml
To destain: incubate the stained gels in the destaining solution for > 30 min on the orbital shaker.
2003 09 26 – Julius